Wednesday, June 23rd, 2021
10:00AM - 11:30AM EDT
Catherine Verfaillie, Leuven University
Anne Marie Vinggard, National Food Institute, Technical University of Denmark
"Multicellular 3D liver models based on hiPSC-derived liver cells"
Presented by Catherine Verfaillie
To address how toxic insults affect hepatocytes as well as non-parenchymal cells, murine or rat models are often used. However, interspecies differences between humans and rodents do not always allow extrapolating findings in animal models to the human patient. Therefore, human hepatoma cell lines with or without stellate cell, monocyte, and/or endothelial cell lines can be used. In addition, and more relevant, primary human liver cells can be used. However, It has long been established that when cultured on stiff culture plates, primary human hepatocytes (PHHs) very quickly lose functionality. This is also the case for hepatic stellate cells (HSCs) that become quickly activated, and liver sinusoidal endothelial cells (LSECs) that very quickly lose their typical sinusoidal endothelial characteristics.
"Novel human pluripotent stem cell-based assays to predict developmental toxicity"
Presented by Anne Marie Vinggard
As women are exposed to a plethora of chemicals during pregnancy, it is important to evaluate their safety for the developing embryo. For this, human based models are needed for enabling reliable and large scale risk assessment1. Human induced pluripotent stem cells (hiPSC) are an excellent tool to model embryonic development as they can form embryoid bodies (EBs) that mirror the early embryo in many structural and functional aspects, and can undergo differentiation programs that reflect molecular mechanisms during embryonic development2. Here, we present our recently developed assays for predicting developmental toxicity using hiPSC.