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Uncle Seminar Series Part 2: The Amazing Blue Shift: what full spectrum fluorescence data can tell you about your protein
Protein denaturation measured by intrinsic fluorescence most commonly demonstrates lower energy fluorescence emission and a reduced fluorescence intensity as aromatic residues are increasingly exposed to solvent. This result is seen as a ‘red shift’ in fluorescence emission spectra since lower energy emissions result in fluorescence at longer wavelengths. Although this pattern is common, it is not exclusive, as occasionally a ‘blue shift’ is observed – a result that stems from denaturation resulting in aromatic residues becoming less solvent exposed. In this webinar we will explore the molecular and biophysical origins of blue shifts, provide concrete blue shift examples, and demonstrate how Uncle tools can be used to find and characterize blue shifts.

Jun 30, 2020 08:00 AM in Pacific Time (US and Canada)

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