Flow cytometry has been applied for decades in diagnosing hematologic malignancies, however, mainly for lymphoid malignancies and acute leukemias. During the last decade flow cytometry emerged from a rather limited method in MDS used only for the enumeration of myeloid progenitors cells to a sophisticated tool capable of identifying specific MDS-related antigen expression patterns which are useful for diagnostic application in patients with suspected MDS. As a result, while not yet conidered diagnostic in the 2016 revision to the World Health Organization classification of
myeloid neoplasms and acute leukemia, flow cytometry is recommended the diagnostic work up of patients with suspected MDS by the 2013 Recommendations from the European LeukemiaNet on Diagnosis and treatment of primary myelodysplastic syndromes in adults. This webinar will demonstrate the principles of flow cytometry in MDS, discuss the standards published on this topic and provide examples of the respective analyses.