International guidelines for the flow cytometric evaluation of peripheral blood for Sezary syndrome / mycosis fungoides. Fiona Craig, MD, Mayo Clinic Arizona
Flow cytometry has a well-established role in the detection of peripheral blood involvement by Sezary syndrome / mycosis fungoides (SS / MF). Recognition of the characteristic CD4(+), CD7(-), CD26(-) T-cell immunophenotype led to early incorporation of flow cytometric parameters into diagnostic criteria for overt disease, such as CD4:CD8 ratio, and proportion of CD4(+), CD7(-) and CD4(+), CD26(-) T-cells. However, a recent multi-institutional survey by dermatologists demonstrated significant variability in the assays offered by different laboratories and the information included in flow cytometric reports. Therefore, an international group of cytometrists was convened to establish guidelines for the flow cytometric evaluation of peripheral blood for SS and MF, including identification and enumeration of immunophenotypically abnormal cells. This webinar will discuss these guidelines, including assay design, optimization and validation, and illustrate some of the common pitfalls in the flow cytometric evaluation of peripheral blood for SS and MF.