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Uncle Seminar Series Part 3: Combining tools for new insights: fluorescence, SLS, and DLS for formulation and antibody development
Simultaneous detection of orthogonal intrinsic fluorescence and static light scattering (SLS) data during a thermal ramp experiment produces original insight on protein behavior not available through other means. The two detection methods align protein conformational and colloidal stability data to link together unfolding and aggregation information on the same sample. Gathering size data from dynamic light scattering (DLS) gives further quantitative information on sample quality before an experiment and aggregation state after an experiment. This talk will explore a few cases studies on how the synergy of Uncle’s three detection methods produce helpful insight in antibody and formulation development.

Jul 9, 2020 08:00 AM in Pacific Time (US and Canada)

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