Oxygen influences multiple physiological parameters within cells. As such, oxygen concentration should at least be considered when planning cell culture studies. In addition to monitoring and controlling for CO₂, humidity and temperature, scientists should consider oxygen levels. Assessing oxygen concentration for all in vitro studies thus improve both scientific reproducibility and relevant outcomes by removing a potential confounding factor.
This webinar will build on these concepts and discuss the importance of oxygen in cell culture. Dr. Pansters will describe better method for defining O₂ concentration, O₂ monitoring and controlling options. He will also provide a brief overview of relevant scientific publications. Participants will gain a better understanding of how accurate oxygen regulation will contribute to improved reproducibility of their experimental work. Finally, participants will learn what next steps exist to improve future research outputs.
Topics discussed in this webinar include:
- The meaning of hyperoxic, normoxic and hypoxic conditions at the cellular level
- How hyperoxic conditions affect the HIF1 transcription factor and its many downstream effects
- How oxygen is beginning to play a larger and larger role in cellular labs
- The future of hypoxic/normoxic cell culturing in research