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Preparing health workers for COVID-19: Laboratory diagnosis - Shared screen with speaker view
Perle Laouenan-Catchpole
33:37
Welcome to todays webinar. Please share your questions in the Q&A panel.
Harriet Natabona
36:41
Harriet Natabona from Jomo Kenyatta University of Agriculture and Technology, Kenya
Joel Andwilege
37:42
Hello everyone. Joel Andwilege Intrahealth international/Tanzania
Edward Chilolo
42:14
I suggest that at the end of this meeting we get the presentation of the meeting
Magreth Antony
01:02:52
Thank you very much Prof.Misinzo for a nice and very knowledgeable presentation...by Magreth Antony
Atunga Nyachieo
01:04:51
Thanks Prof. Misinzo for a wonderful presentation. Dr. Atunga Nyachieo, from Kenya.
Yegon Kibet
01:05:03
Yegon Kibet from KEMRI
Annamary batoleki
01:05:49
Thank you Prof. Misinzo for the good presentation
Elifuraha Barnabas
01:06:34
Hi, My name is Elifuraha Barnabas from Sokoine University of AgricultureThank you prof Misinzo for knowledgeAs you explained, testing for the COVID virus should be an easy process. However I am not sure how Africa, particularly Tanzania is well prepared to handle infectious material. Given the biosecurity challenges in Africa, we need to invest more on handling biohazard material
Yegon Kibet
01:06:36
Thank you Prof. Misinzo, learned alot from the presentation.
Edward Chilolo
01:06:49
Thank you prof Misinzo for this a simplified and nice presentation
Professor Misinzo
01:07:08
@Barnaba, biosecurity is an issue during sampling and nucleic cid extraction. You have to wear a full PPE. After that, practice BSL-2
Peter kidenya
01:07:09
Thank you Prof Misinzo for wonderful presentation /Peter-Tanzania
Galaxy A50
01:07:24
thanks for good presentation Prof Misinzo by Dr Wambura from Tanzania
Annamary batoleki
01:10:55
Hello Elifuraha Barnabas. Tanzania has been trained laboratory Scientists from all over the region on handing the infectious material based on Biosafety and Biosecurity regulations
Professor Misinzo
01:12:37
Regarding preservation of RNa in the absence of liquid nitrogen or cold chain. Please collect using Trizol or lysis buffer to kill the virus and preserve RNA. Primestore or RNA Later are reagents that are also sold commercially. They will preserve the RNa and still enable you test using qRT-PCR. This will not be good for cell culture though
Professor Misinzo
01:15:02
Regarding the target genes in SARS CoV-2: some of the genes are conserved in all betacoronaviruses while some are very specific for SARS CoV2. For SARS CoV-2 there is at least more than one target gene, to take care of possible miss in case a mutation occurs. With the access to instant genome sequencing, these mutations can be easily located to inform change in strategy for testing and interpretation of results
Professor Misinzo
01:15:57
Samples: Blood is good for serology. Oropharyngeal and nasopharyngeal swabs, are the samples of choice, and whenever possible an expectorated sputum
Peter kidenya
01:16:02
currently there PRIMESTORE MTM a micro tubes with solution which safely inactivate the organism and preserves the RNA for future , you can travel with samples for as long as 30days at 40C without cold chain system regardless the RNA instability
Atunga Nyachieo
01:16:09
Prof. Misinzo, given that Tanzania is a large country how many testing labs are designated for c-19 testing?
Atunga Nyachieo
01:16:32
Also have started mass testing?
Professor Misinzo
01:16:35
Kidenya: Thanks. This rhymes with my answer: Regarding preservation of RNa in the absence of liquid nitrogen or cold chain. Please collect using Trizol or lysis buffer to kill the virus and preserve RNA. Primestore or RNA Later are reagents that are also sold commercially. They will preserve the RNa and still enable you test using qRT-PCR. This will not be good for cell culture though
Professor Misinzo
01:17:29
Laboratory designations: many labs have qRT-PCR machines and ready for commissioning based on need and volume of samples or risk in certain area
Professor Misinzo
01:19:28
Sensitivity: each kit has a limit of detection (LOD) and this varies with the target gene too. Overall, qRT-PCR is highly sensitive with some tests detecting as low as 3 RNA copies per 1000 uL
Atunga Nyachieo
01:19:36
Prof. Misinzo, there are discussions on use of serology for diagnosis of covid -19, what is your opinion on this?
Ephrasia Hugho
01:21:37
Is there a plan to follow up patients after been discharged from hospital? Or any possibility of disease recurrence later? on
Professor Misinzo
01:22:15
Thank you all making time to attend. I see tests from all over and from some familiar people. Greetings to people in Ndanda, TAWIRI, NM-AIST
Silver Timoth
01:23:04
Thank you so much Prof for sharing with us
Elifuraha Barnabas
01:24:21
I am calling upon African scientists to help Africa by first doing epidemiological study to determine the exact prevalence of disease. This is key for managing the crisis
Gildas Hounmanou
01:26:08
Thanks for presentation
Atunga Nyachieo
01:26:54
Prof. Mwau, thanks for nice presentation
Malewo
01:27:05
Thank you Dr. for very nice presentation
Alexander Mzula
01:27:13
Thanks prof Misinzo for showing our diagnostic capacity at molecular level as well as human resorce capacity
Peter kidenya
01:27:14
Thank you Prof for this presentation
Henry Mokiwa
01:27:20
we can, we will and we Must defeat covid 19
Dennis Massue
01:27:36
Thank you Prof Mwau and Prof Misinzo for the good presentations
Gildas Hounmanou
01:27:57
In this kind of situation, for the Mass test is it really possible to use serological test? I think when available RDTs test and PCR are more interessting! Your opinion please
Malewo
01:29:35
I liked the idea of coming up with other options especially when supplies are not available
Victor J Simon
01:29:40
Prof .Misinzo how are we prepared to meet an increased demand for clinical testing of the disease in Tanzania ,as we are looking forward to have an increase in reported cases for the coming days,as of now we only have two labs capable of testing of the disease,,Victor Simon
PAUL JOHN
01:29:48
are this virus can be transmitted through blood???, e.g in blood transfusion
Edward Chilolo
01:30:26
Thanks the profs and PharmAccess for preparing this learning platform. I hope you are going to do another meeting in coming days-Chilolo-Tanzania.
Yegon Kibet
01:31:11
Thank you Prof. Mwau for painting a clear picture of where as a country we stand, where we need to go and how to work our way there.
Perle Laouenan-Catchpole
01:33:13
For resources helping you prepare for COVID-19 you can visit www.safe-care.org
Isack Kaniki
01:33:18
Thanks Prof Mwau for your presentation and significant control strategies in your country .
Perle Laouenan-Catchpole
01:33:57
For questions not answered today please feel free to email info@safe-care.org
Professor Misinzo
01:34:11
Serology will also be able to inform policy on whether to have lock down or not. If most population is exposed, then there is no need for a lockdown. We could practise and intelligent lock down
Professor Misinzo
01:34:20
You are right Gildas
Perle Laouenan-Catchpole
01:34:22
www.safe-care.org
Professor Misinzo
01:34:28
As for the probes: Most kits are coming with FAM, and one has VIC. The quencher used is black berry quencher and black hole quencher 1
Professor Misinzo
01:34:37
Serology will also be able to inform policy on whether to have lock down or not. If most population is exposed, then there is no need for a lockdown. We could practise and intelligent lock down
Professor Misinzo
01:34:53
Thank you Dr Chilolo
Hassan Katuli
01:34:57
Thank you for the meeting, it was great and mind opening regarding covid-19.
Davis Kuchaka
01:35:08
Thank you a wonderful presentation