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ONCO MS-19 - Shared screen with speaker view
Mohit Kumar Jolly
32:16
Is it possible to track whether escapees can convert over the timescale of drug treatment? Is this switching coupled to cell division?
Mohit Kumar Jolly
32:38
convert to non-escapees*
levine
33:01
Can you comment on whether you also saw what was reported in the Quaranta lab that they called “an idling population” ie a constant total population after drug tolerance emerged
Joshua Scurll
34:04
i can unmute to ask a Q
Kishore Hari
34:20
yes
Yogesh Goyal
34:37
I’ll ask on mic as well!
Mubasher
01:01:18
wonderful talk Yogesh!
Jaewook Joo
01:02:11
do you vision that the epigenetic phenotypes alvan be modeled by different genetic networks or the same netowkr with different parameter values?
Jaewook Joo
01:03:54
also did you observe the switching during the experiment
Yogesh Goyal
01:05:09
re: switching, yes, we have verified with imaging the origins of new states only in low does but not high dose.
Jaewook Joo
01:26:01
I have heard that EMT is not a jump between two discrete states, but continuous transition along continuous state spectrum. Do you have any comment about that view?
Mohammad Zahid
01:26:57
We don’t see your screen right now
Eduardo
01:27:32
show screen please
Jenny Alfaro
01:29:50
Hi doctor, I'm new working with BNs and EndMT, which is the most difficult step to determinate the parameters based on RNA-seq in single cell? (sorry if I'm not clear)
Mohit Kumar Jolly
01:30:14
Hysteresis in EMT (as seen at single-cell proteomics, bulk RNA-seq, morphological space) also argues against continuous spectrum and favors a more discrete state-space, right?
Mohit Kumar Jolly
01:41:16
Coming to the question of necessity and sufficiency: Non-heritable drug-tolerant states can be sufficient for resistance (Einar). Yogesh, Sabrina: Do you think non-heritable drug-tolerant states are necessary for long-term resistance?
Joshua Scurll
01:43:59
How would you envisage estimating μ and ν for a patient?
Yogesh Goyal
01:46:31
Mohit, It’s a bit hard for me to imagine non-heritable states (random fluctuations?) is necessary for drug resistance. Many people have found that plasticity underlying drug resistance or even metastasis is heritable in many cases (including a recent beautiful paper from Trevor Bivona lab this year in Science)
Yogesh Goyal
01:46:48
Heritable but not permanent.
Jaewook Joo
01:46:51
Sounds like that tumors evade therapy much more likely with phenotype switching than genetic mutation alone. As Dr. Gatenby advocated strongly, our current therapy paradigm needs to be dramatically shifted from tumor control to tumor maintenance.
Mohit Kumar Jolly
01:50:58
Great; thanks Yogesh!
Yogesh Goyal
01:52:00
Thanks Mohit and Kishore for this great mini symposium!