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CDEV CT-7 - Shared screen with speaker view
Suzanne Sindi (She/Her)
29:12
@Jordan is here! :D
Daniel Cruz
31:07
I will type it
Suzanne Sindi (She/Her)
31:11
Daniel: Please type your question! :)
Daniel Cruz
32:59
So I may have missed it, but it seemed like you were using some kind of statistical average to claim that budding cells were more connected than non-budding cells (I think). If that is the case, then are you looking into something less statistically-based to connect budding to these larger, emergent feature of connectivitiy?
Mikahl Banwarth-Kuhn (she/her/hers)
36:00
Yes, we used statistical averages of graph properties to first understand WHETHER budding division made a difference in the spatial organization of the colonies. Because no one had looked at this specifically before.
Kevin Tsai
37:01
@Mikahl Nice talk! Just a couple of questions: (1) Is there a max limit of how many generations a cell can bud? (2) Is apoptosis included in the model?
Mikahl Banwarth-Kuhn (she/her/hers)
38:49
@Daniel- so we sort of answered the question of whether budding impacts the spatial organization and the answer was that locally mother and daughter cells remained closer together for longer. But what we learned is that the sort of sectoring behavior we wanted to recreate (which we called a more global property) was more impacted by nutrient limitation than the budding division.
Mikahl Banwarth-Kuhn (she/her/hers)
40:03
@Daniel- My suspicion is that the budding will matter more once we fully consider aggregate dynamics. But I’d like to have more of a back and forth with you on what kinds of none statistically based methods you have in mind to asses these changes.
Mikahl Banwarth-Kuhn (she/her/hers)
41:19
@Kevin- Yes, our cells have budding scars so they can’t bud in the same place twice and also in the timeframe of our simulation they are not exceeding what has been seen in experiments in terms of how many buds a cell can produce.
Kevin Tsai
42:31
@Mikahl I see. Thanks.
Mikahl Banwarth-Kuhn (she/her/hers)
43:28
And we do not have apoptosis in our model because we only simulated 24 hours and to our knowledge the average lifetime of a cell exceeds that timescale.
JOSUE MANIK NAVA SEDEÑO
47:28
Can we find this work in any paper?
JOSUE MANIK NAVA SEDEÑO
47:56
Or is it a thesis?
Benjamin Brindle
48:17
@Josue This work was part of my thesis
Suzanne Sindi (She/Her)
48:29
Coimbra is one of the most beautiful places I’ve ever been :) <3
JOSUE MANIK NAVA SEDEÑO
49:01
It would be really nice to read the mathematical details, that's why I was asking :)
Dr. Miranda Teboh-Ewungkem
49:36
Josue, it is being updated for a paper.
JOSUE MANIK NAVA SEDEÑO
51:13
Alright, I'll be looking forward to it then, thanks
Suzanne Sindi (She/Her)
01:03:42
@Marcos: Very neat talk! Is this work published? (I’d appreciate a reference.)
Marcos Gouveia
01:06:15
Hi Suzanne thank you :) We are currently working on a manuscript for the work I showed. There is a seminal paper called "Force at the Tip" in PLOS Comp.Biology which introduces the model and studies the effects of cell proliferation on angiogenesis
Suzanne Sindi (She/Her)
01:14:48
Thanks Marcos - I’ll follow-up with an email :)
Marcos Gouveia
01:15:18
awesome :)
Suzanne Sindi (She/Her)
01:18:36
Nice session! Great job everyone!!!
Chris Miles
01:18:38
awesome session! thanks to all the speakers for all the cool talks!