Zoom Logo

CDEV MS-6 - Shared screen with speaker view
Roeland Merks
33:43
What do you see if you add the self-generated gradients? ;-)
z.bell@newcastle.ac.uk
33:49
Was the diffusion coefficient independent of the position or did it alter depending on the density of the area that the cells were in?
Yue Liu
35:44
Are there any results for limiting cases, such as for very high obstacle density
z.bell@newcastle.ac.uk
36:48
Thank you :)
Yue Liu
37:59
Thank you
Roeland Merks
38:16
thanks!
Roeland Merks
40:32
we cannot see the lower part of the slides :-(
Renske Vroomans
01:00:52
Great talk Lisanne, I have a question (shall I ask it live?)
Rui Travasso
01:00:55
Wonderful work!!! Thank you :) What is the timescale associated to the oscillations? Can you control the timescale (is it easy?)? Have you tried for the cell to move along a 1D fibronectin path? Could you see movement inversion in that situation?
Roeland Merks
01:00:56
How does the flexibility of the pillars affect cell motility
Roeland Merks
01:01:11
And how is this predicted by the model
Roeland Merks
01:01:41
And great work :-)
Renske Vroomans
01:02:35
I was going to ask if you were going to look at collective migration, but you will, that is very cool! Does the Rac/Rho system signal across membranes between cells, or are they isolated?
Lisanne Rens
01:04:59
@Rui, I haven't tried movement of a cell along a 1D path/wire or something like that. That's a great idea. For now I've only done static 1D simulations
Lisanne Rens
01:05:46
@Roeland, thanks! Nice question, haven't explored that but is an interesting next step. The effect of pillar flexibility could be described in the adhesion dynamics equation
Roeland Merks
01:06:19
Yes, would be cool to couple it with the FEM-CPM framework as well!
Lisanne Rens
01:06:54
@Renske, yes that would be very exciting right, the collective migration. The idea is that at cell-cell adhesions, the cells can signal to active Rac/Rho in neighbouring cells
Renske Vroomans
01:08:06
ah interesting -- look forward to seeing that!
Roeland Merks
01:21:18
In Lisanne’s iScience 2020 model we found that stochastic extensions of the membrane were a key contributor to cell spreading. It creates a kind of ratchet effect....
Roeland Merks
01:21:53
… the cell extends stochastically, and that gets ‘stuck’ to the substrate with probability dependent on substrate stiffness. To what extent is this effect related…
Roeland Merks
01:22:08
… to the membrane unfolding effect that you discussed?
Roeland Merks
01:22:19
(and thank you, great talk :-)
Bindi Brook
01:22:37
Thanks for a very nice talk! Apologies if I missed this but do you assume that the membrane is linearly elastic? Wouldn’t a non-linear strain stiffening effect have an effect on the results?
Roeland Merks
01:24:10
yes
Roeland Merks
01:24:17
yes!
Lisanne Rens
01:24:27
perhaps the time evolution of the forces (which I am not sure Magda included) due to substrate stiffness is a crucial thing that helps for cell spreading
Lisanne Rens
01:24:40
which we had in our iscience paper too
Roeland Merks
01:25:35
Yes. thanks, that might be related indeed.
Roeland Merks
01:25:54
thank you!
francois el-daher
01:48:58
Great talk! Have you considered to add some plasticity/deformability to ECM? Meaning that the nodes position would be changed irreversibly or with a long relaxation time by the moving cell. Maybe using a Maxwellian model for ECM instead of purely elastic?
francois el-daher
01:51:29
Indeed, thanks!
Kara Allum
01:54:36
Thank you all for a great session :)