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IMMU MS-7 - Shared screen with speaker view
Miranda Lynch
23:34
Can you comment on the time scale of the morphing between the pro-capsid form and the mature virion form?
Mike Pablo
30:52
Would it be possible to fluorescently label only a small fraction of the molecules of interest, so that you can look at higher concentrations relevant for assembly but still have very single bursts of fluorescence?
Miranda Lynch
32:46
The Forster distances of 55 angstroms in the FRET seems quite large. Could you give a rough idea of the 'size' of an individual monomer, and the size of the final assembled virion?
Miranda Lynch
36:54
Can you get cryoEM structures of the mutant forms you used? You showed that the mutants still assemble into mature structures, but it would be interesting to see if any structural changes take place.
Miranda Lynch
37:38
Yes, thank you!
Carolyn Teschke
59:16
Thank you SiYu!
Siyu Li
59:45
Thank you!
Miranda Lynch
01:15:28
The retained enzyme in the wiffle ball constructs, you mentioned might be retained via aggregation. Have you confirmed the aggregability of the enzymes you are using? Do they naturally self-associate? Do we see aggregates of the proteins outside of these nano-constructs?
Carolyn Teschke
01:20:34
Have you looked at scaffolding protein exit with your charge mutants?
Miranda Lynch
01:23:59
Thank you @Trevor, wonderful work. Does the disordered portion in the non-truncated components assume induced structure in the final assembled particle?
Trevor Douglas
01:28:00
Hi Miranda - thanks. We have no information about the ordering of the scaffolding protein inside - unfortunately.
Roya Zandi
01:38:16
Is the stress accumulated around the pentagonal defects?
Miranda Lynch
01:47:40
Great session, thanks to the organizers!
reidun
01:48:00
Thank you so much for attending and your great questions!
Siyu Li
01:48:51
Thanks everyone for the great session!
Carolyn Teschke
01:48:54
Thank you for the great session!
Stephen E. Moore
01:49:05
thank you